For binding analyses, membranes were diluted in assay buffer (50 mM HEPES, 5 mM EGTA, 5 mM MgCl 2 , 0.005% Tween 20, pH = 7.4) to a final concentration of 0.2 mg/ml of protein and distributed (3 g/well) to 96-well OptiPlates
Since these guidelines are preliminary, researchers should conduct initial dose-finding studies to identify the optimal range for their specific models
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