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Cells were lysed in PBS/Triton X-100 (0.5%) for 60 min

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Improper reconstitution can be catastrophic for your research

GST Tag Interference with Protein Function Challenge : In some cases, the GST tag can interfere with the function of the target protein, particularly if the protein's active site or interaction domains are close to the fusion point

It means there's a considered range, and the honest move is to pick a defensible point inside it and tell you what it is