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Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) According to the protocol, total RNA was extracted from the large intestine with TRIzol (Life Technologies Japan Ltd., Tokyo, Japan) using a Dounce Tissue Grinder (DWK Life Sciences, LLC, NJ, USA), and 500 ng of RNA was reverse transcribed to complementary DNA using the Super Script III First-Strand Synthesis Super MIX kit (Life Technologies Japan Ltd.)

Musial-Bright et al., 2011)

So, instead of thinking of sermorelin as a peptide that increases muscle mass, its more accurate to think of it as a peptide that stimulates the production and secretion of endogenous HGH levels, which in turn, may lead to increased muscle mass

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