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Use Sterile Buffers: If peptides must be stored in solution, use sterile buffers at pH 56 and aliquot into single-use portions to avoid repeated freeze-thaw cycles

Any lyophilized drug intended for repeated withdrawals from the same vial typically specifies bacteriostatic water as the diluent

Monitor liver enzymes (ALT, AST) as semaglutide has rare reports of liver injury

Low cell density (only 1-5% of cartilage volume, rest is matrix)

Since the first-line intervention for obesity is typically lifestyle change(s) 4 , this study purposefully combined LD switch with 5A-1MQ treatment

Multicenter trials would provide sufficient power to detect clinically meaningful effects and assess safety across diverse patient populations