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They were seeded onto 6-well cell culture plates and transiently transfected with different GLP-2R constructs using Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA, USA)

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ESCRT is a highly conserved transport system commonly found in yeast and other eukaryotic cells, and it is programmed to transport ubiquitinated proteins into lysosomes 80

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[DOI] [PMC free article] [PubMed] [Google Scholar] 48.Talbert ME, Langefeld CD, Ziegler J, et al