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Samples were allowed to cool, and 1.375 ml purified water with a resistivity 18.2 M cm from a Milli-Q system (Merck Millipore) was added to make up to a final volume of 1.5 ml

During the program, participants in the last two classes have seen their resting heart rate decreaseby an average of almost 7 beats and an increase in their HRV of 26 points

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Isolation of cardiomyocytes, endothelial cells, and fibroblasts from mouse hearts ECs, FBs, and CMs were isolated from mouse hearts as previously described, with modifications 62