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Trinit, Romano di Lombardia, (Bergamo), Italy]

10.1038/mp.2010.121 261

Samples (whole cells or isolated mitochondria) were freeze-thawed three times, and the lysates filtered through 10-kDa molecular mass cutoff devices (Amicon, Millipore) at 12,000 g for 15 min at 4 C

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Routine monitoring of serum calcitonin or using thyroid ultrasound is of uncertain value for early detection of MTC in patients treated with Saxenda