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Niacin causes vasodilation and flushing due to activation of the GPR109A receptor, while niacinamide does not

Briefly, cells were lysed using the RIP lysis buffer provided in the kit, and cell lysates were incubated with magnetic beads pre-coated with either anti-SYNCRIP antibody (R5653, Sigma-Aldrich) or purified mouse IgG (5415, Cell Signaling Technology) as a negative control

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