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Cells were washed with PBS and fixed with 4% paraformaldehyde (PFA) for 10 minutes at 4C, permeabilised for 15 seconds in a liquid nitrogen bath, blocked in 1% BSA / PBS for 1 hour at room temperature, incubated with D4H*-mCherry (1:25 in 1% BSA / PBS) for 2 hours, washed with PBS and post-fixed in 4% PFA for 10 minutes at room temperature
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Barnes says
Consequently, the accurate attribution of the obtained peptide effects, particularly for skin defect healing (where unlike uncertain healing attribution of other peptides which are given with carriers (4-9), BPC 157 is given alone and thereby acts directly), the therapeutic success of BPC 157 over the external fistulas model (10-12) and now in duodenocutaneous fistulas further emphasized the real healing congruence of the skin healing and other gastrointestinal tissues healing
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