The absorbance for each condition was measured, and the percentage of cross-reactivity was calculated using the following equation, adapted for non-competitive sandwich immunoassays 53 : Cross-reactivity (%) = [(OD of cross-reactant analyte)/(OD of target analyte)] 100
Reactions products were separated by native gel electrophoresis in 6% polyacrylamide TB (89 mM Tris, 89 mM Boric Acid) gels or 5% polyacrylamide SB gels (10 mM NaOH, 38.5 mM Boric Acid, pH 8.5) at 100 V for 45 min
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Major manufacturers & their revenues, percentage splits, market shares, growth rates and breakdowns of the product markets are determined through secondary sources and verified through the primary sources
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