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After 2 hours of incubation at 37C in a 5% CO 2 incubator, 1 M monensin and 10 g/ml brefeldin A were added to the culture and the cells were incubated for another 4 hours

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Mild effects include headache, flushing, light-headedness, injection-site redness, or temporary lethargy

[4] Patients on drugs for pulmonary hypertension (e.g., PDE5 inhibitors or guanylate cyclase stimulators) could theoretically see diminished effects if MB is introduced, due to MBs antagonism of NO signaling