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After incubation, 50 L of K 3 [Fe(CN) 6 ] (2 mM) was added and the absorbance at 680 nm was recorded with a BioTek synergy 4 Multi-Mode Microplate Reader

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It is likely that a redox reaction between reduced ascorbic acid and hematin generates free radicals, which in turn leads to the degradation of the porphyrin ring