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Specific microbial species (e.g., Ruminococcus gnavus and Bifidobacterium ) may participate in SLE pathogenesis via antigenic molecular mimicry or immunomodulatory metabolite production

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DAB staining and preparation of cultured cells for EM The fixative was removed and cells were washed (five times for 1 min) with 0.1 M sodium cacodylate (Ted Pella, 18851) buffer at pH 7.4 containing 2 mM CaCl 2 on ice and blocked with 20 mM glycine in 0.1 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 for 20 min on ice

Prepare a clean, well-lit workspace with all supplies within reach

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The crew handles it before lunch