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Through timed injections of pharmacological modulators (e.g., metabolic inhibitors, activators or substrates), the assay facilitates a comprehensive investigation of mitochondrial and glycolytic functionality

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Concentration of P, S, Na, K, Mg, Ca, Fe, Cu, Zn, Mn, Mo and B were determined by inductively coupled plasma highly dispersive optical emission spectrometry (ICP-OES) according to the method described by Kalisz et al