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Experimental Protocol A total of 24 rats were randomly divided into four experimental groups (n = 6 per group): control, group B, IR, and IR-BPC157

Subcutaneous: A small injection into the fatty layer just under the skin the same way insulin is given

ADP Ribosylation Factor 6 (ARF6), a part of the RAS superfamily, facilitates high sensitivity to RSL3-induced lipid peroxidation (Ye et al., 2020)

Red puncta (mRFP only, representing autolysosomes) and yellow puncta (overlapping of mRFP and GFP, representing autophagosomes) were counted and presented as number per cell

Dealing with autoimmune concerns

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