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By 6 weeks, there were already measurable decreases in both the intensity and size of these spots

Our approach emphasizes data integrity, structured methodology, and the continuous exploration of mechanisms involved in cellular recovery and biological signaling pathways

The regulation of the VHLHIF pathway is also related to iron metabolism and ferroptosis in ccRCC, as iron levels are significantly higher in RCC cells and the tumor microenvironment than in normal renal tissue (77), and as HIF-1 mediates the cellular upregulation of TfRC and heme oxygenase (HO) expression, promoting cellular iron accumulation (78, 79)

They also oxidize proteins and DNA [63], leading to several structural damage, functional impairments, and genetic mutations that ultimately affect cellular and organismal homeostasis [64]

We validated corresponding cell survival under different conditions with propidium iodide (PI), colony-forming, and 3-dimensional spheroid assays (Fig

Notably, similar results were obtained using ATCC46645 and gliT 46645 strains (data not shown)