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Following 3 h of drug treatments, 5 M of the cell-permeable, ROS-sensitive dye CellROX Deep Red (Invitrogen, Stockholm, Sweden) was added to each sample and incubated at 37 C for 30 min

Rare risks include copper sensitivity or toxicity with prolonged use which is why we monitor copper levels with periodic blood tests

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Stops mutations Stops molecules inert, preventing their destructive effects Vital for both phase I & phase II liver detoxification Induces normal p53(tumor suppressor) activity by redox modulation, which induces apoptosis in tumor cells Detoxification is another major function

Store reconstituted vials properly (refrigerated, protected from light), and respect expiration dates to ensure potency and sterility

This overview presents the scientific evidence behind GHK-Cu