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Bacterial pellets were obtained by centrifugation for 15 min at 3,220 g and pellets were resuspended in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 (pH 7.5)

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Unlike gradual vision loss where patients can adapt over time, NAIONs acute onset leaves victims unprepared for a world designed for sighted individuals, necessitating immediate accommodations, vocational rehabilitation, and extensive support services

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