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Radiotherapy inhibits tumor growth by generally inducing tumor cell apoptosis, senescence, autophagy, necrosis, or mitotic catastrophe through inflicting extensive DNA damages (Chen and Kuo, 2017)

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The excision of the SAT1 flipper cassette was induced by inoculating 5 mL of YPD broth, containing 2% maltose (without nourseothricin), with the nourseothricin-resistant strains and incubating them in a rotary shaker at 30 C overnight