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Gln is recognized as the most abundant non-essential amino acid in human plasma [2], with a concentration reaching 0.8 mM under physiological conditions and the potential to comprise up to 40% of the total intracellular amino acids content [3]

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Further, with the increase in the incubation time we observe an intense C-D band from the untreated cells whereas negilibile dynamic changes in the CD band intensity with time is observed for bacteriostatic antibiotic treated cells

[3] Fernndez-Lzaro, D., Domnguez-Ortega, C., Busto, N., Santamara-Pelez, M., Roche, E., Gutirez-Abejn, E., & Mielgo-Ayuso, J