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The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212
However, if your calculated volume exceeds 1 mL, you may need to split the dose into two injection sites during the same administration
Cai M, Hruby V J
Any diet restriction requires careful consideration to ensure the appropriate supply of essential nutrients
Toxicon 145, 3239
Research Diets, D12108 C, 1.25% cholesterol) or an ALC-supplemented diet (300 mg/kg, Changzhou Shuiyushuer Company, Changzhou, China) for 4 weeks ( n = 6 mice per group)
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