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Cell viability was quantified using the Cell Counting Kit-8 (CCK8) assay

Grey, Vijaylaxmi, et al

Therefore, we estimated a total annual burden of 400,464 hours for all hospitals (7,416 hospitals 54 hours) at a cost of $36,519,350.40 (7,416 hospitals [($128/hour 2 hours) + ($87.52/hour 40 hours) + ($97.30/hour 12 hours)])

Currently, most of the available data comes from in vitro experiments and studies involving rats and mice, not large-scale human trials

Plus, injections give a stronger and faster effect

The disease-associated r(GGGGCC)n repeat from the C9orf72 gene forms tract length-dependent uni- and multimolecular RNA G-quadruplex structures