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3.2 Expression of GST fusion protein One of the driving forces behind the development of the GST fusion system is that the GST protein accumulates in the cytoplasm (1) and, due to the large size of this affinity tag, it is expected that the solubility of the GST moiety would usually propagate to the fusion partnerthereby eliminating the need for lengthy purification schemes for insoluble proteins that usually involve denaturants

This is a subtle but devastating failure point in research, leading to inconsistent or null results that are impossible to troubleshoot