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Surface receptor probe was cleaved by incubation in 100 mM 2-mercaptoethane-sulfonic acid sodium salt (Mesna) in 1X TAE buffer, pH 8.6 or incubated 1X TAE buffer alone for 5 minutes and then washed and imaged in HBSS using a custom-written high content analysis software in Micro-Manager in a Cairn Research widefield microscope with Nikon Ti2, CoolLED light source and 20X phase contrast objective for both epifluorescence and transmitted phase contrast with 9 ROI acquired per well