Analyses were conducted using available values within the TriNetX platform, and patients with missing covariates were excluded from the relevant variable-specific analyses
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Brains were snap-frozen in isopentane and the OB ( n = 10) or LH ( n = 8) was cut into 18 or 30 m-thick sections respectively, using a cryostat (Leica Biosystems)
Anorectic estrogen mimics leptin's effect on the rewiring of melanocortin cells and Stat3 signaling in obese animals