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At 2 h after plating, the plating medium was replaced with fresh culture medium consisting of DMEM supplemented with 1 mM sodium pyruvate, 100 units/ml penicillin, 100 g/ml streptomycin, 0.2 % (vol/vol) of bovine serum albumin fraction V (instead of FBS), 100 nM dexamethasone, and 1 nM insulin (SigmaAldrich #I9278)

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DISCUSSION Trichotillomania, which was previously included in the obsessive-compulsive disorder (OCD) spectrum, was reclassified as an OCD-related disorder (OCDRD) in the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5)

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