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In T2D patients (n = 25, age 2565), consumption of 1 g L-citrulline twice a day for 4 weeks reduces the T2D-induced increase in arginase activity and increases plasma nitrites, effects corroborated by improvement of ACh-induced relaxation in ex vivo mouse aortic rings incubated (24-h) in low or high glucose-containing media and increases in NO production in ECs treated with high glucose (Shatanawi et al., 2020)

Eluted pure CrAT and CPT2 proteins were dialyzed against above phosphate buffer without any imidazole and then concentrated and kept frozen in small aliquots at 80C

doi: 10.3122/jabfm.2021.03.200502

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10.1016/j.lfs.2020.118457 59 LiuL.ChenJ.WangL.ChenC.ChenL