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RNA was then extracted using a SteadyPure Universal RNA Extraction Kit (Accurate, Hunan, China) in accordance with the manufacturers instructions

(14) Symptoms such as nausea, vomiting, and lack of appetite, which can all occur with appendicitis, arent necessarily predictive of appendicitis in children, according to a 2007 study

The challenge is elucidating the mechanisms by which microbial metabolites affect brain function, which stems from the unclear rate at which some microbial molecules are transported to the brain

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PMID: 26784461