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HO-1, the rate-limiting enzyme in the production of bilirubin, catalyzes the oxidative cleavage of the heme molecule to form biliverdin and carbon monoxide (CO)

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Secondly, the redox status of the cell affects many of the enzymes in one-carbon metabolism including MATI, MATIII, MS, BHMT, as well as CBS and GCS in the transsulfuration pathway, and therefore one cannot evaluate GSH metabolism without including the methionine and folate cycles

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