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Total glutathione lysis reagent and GSSG lysis reagent, which contains alkylating agent N-ethylmaleimide (25 mM) were prepared following manufactures protocol and added to respective wells along with blank for background

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The differentially expressed genes (DEG) were then analysed using the DESeq2App

These medications help: Slow stomach emptying, leading to increased feelings of fullness Reduce appetite and food intake by acting on brain centers that control hunger Improve insulin secretion and sensitivity Lower blood sugar levels, especially after meals Promote weight loss through multiple mechanisms Mounjaro (tirzepatide) is actually a dual GIP/GLP-1 receptor agonist, meaning it acts on two hormonal pathways related to blood sugar control and appetite regulation, which may explain its particularly strong effects on weight loss

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