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If symptoms continue or intensify, its worth revisiting your treatment plan with your provider to explore possible adjustments

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doi: 10.1371/journal.pone.0089208 39 ShihC.-C.LeeH.-H.ChenT.-L.TsaiC.-C.LaneH.-L.ChiuW.-T.et al

Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution

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