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Panc-1, L3.6pl, and KPC cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS), penicillin, and streptomycin (Invitrogen)

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TB-500 contributes critical cytoskeletal and cellular migration mechanisms that enable fibroblasts to respond effectively to the synthetic and migratory signals provided by GHK-Cu and BPC-157. Research demonstrates that TB-500 treatment extends the anagen (growth) phase duration, increases cellular proliferation, and enhances the structural organization of newly synthesized ECM through its effects on actin dynamics and cellular mechanotransduction

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