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Ambitious protocols integrate direct measurements: Electrophysiology LTP (Long-Term Potentiation) hippocampal on CA1 slices, gold standard of synaptic plasticity

Moreover, the C-terminal serine of the peptide was replaced by cysteine via site-directed mutagenesis performed with polyethylene glycol

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Buffer A (95% H 2 O, 5% methanol, 5 mM ammonium acetatepH adjusted to 5.0 with acetic acid) and buffer B (95% isopropanol, 5% methanol) were used

Researchers across France, Germany, Italy, the Netherlands, and other European countries utilize compounds like 5-Amino-1MQ to investigate intracellular communication and biochemical response mechanisms without relying on complex protein structures

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