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In addition, MPO activity was estimated using an MPO Detection Kit (Nanjing Jiancheng Bioengineering Institute, China)

Enzymatic glutathionylation reactions are tightly controlled and highly specific and are considered a major post-translational modification that occurs in response to fluctuations in local redox environments

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Additionally, around 12% of oxygen used in mitochondrial metabolism produces free radicals, which can lead to oxidative stress and even DNA damage [2]

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