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Replacing the lid domain of Candida antarctica lipase B (CAL-B) with homologous domains from Neurospora crassa and Gibberella zeae lipases resulted in improved activity and enantioselectivity toward a racemic mixture of ethyl 2-phenylpropanoate, the hydrolysis of which is an important step in the synthesis of Profen drugs such as ibuprofen

FIGURE 1 Next, to determine the neurotoxic effect of peroxynitrite on cultured SGNs, the cultured SGNs were treated with different concentrations (100 or 200 M) of peroxynitrite for 24 or 48 h, respectively (Figure 2A)

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(1) Participants: adult patients (aged 1880 years) with the similar duration of obesity and with a newly diagnosed T2DM, pharmacologically naive, with a similar state of metabolic balance (similar HbA1c level), not achieving weight loss during previous (at least one year) non-pharmacological treatment for obesity (2) ability to give informed consent, understand and comply with the study procedures

doi:10.1152/ajpendo.00326.2004 7

If it does not dissolve within a few minutes of gentle swirling, set the vial in the refrigerator for 15-20 minutes and try again