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TLR4:Toll-like receptor 4

2.10 Flow cytometry Single-cell suspensions were obtained from a lung section by digestion with collagenase IV (0.25 mgmL 1 ) at 37 C for 30 min, followed by filtration through a 70-m cell strainer and red blood cell (RBC) lysis using RBC lysis buffer (BioLegend) for 2 min at room temperature

Mitsuishi Y, Taguchi K, Kawatani Y, Shibata T, Nukiwa T, Aburatani H, et al

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