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Periodontol 2000 83 , 90106 (2020)

Handle vials and syringes carefully, as they are fragile and may break or cause injury if mishandled

*Correspondence: Chao Liang, [email protected] These authors have contributed equally to this work This article was submitted to Autoimmune and Autoinflammatory Disorders, a section of the journal Frontiers in Immunology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Samples of small volume (150-200L) were thawed under strict cooling conditions (+0C to +2C) within 12-15 minutes in an ice-water bath preferentially without agitation