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For trials that included background therapy with and without metformin HCl, only patients who received metformin HCl were included in the 8-trial placebo-controlled pool

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400,000 B16-F10 murine melanoma cells were injected into the tail vein of C57BL/6 mice

Design a Robust Experiment: Develop a detailed protocol including control groups, appropriate measurement endpoints, and planned statistical analyses