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dilution, 1:2,500) antibodies, and then probed with secondary antibodies conjugated with horseradish peroxidase (both from Santa Cruz Biotechnology, Santa Cruz, CA, USA)

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In the physiological context, our convergent data from intra-OB Ex9, IPGTT and ex vivo OB GLP-1 release experiments indicate local production of GLP-1 in the OB, presumably independently of peripherally derived GLP-1

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