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Cells were lysed in radioimmunoprecipitation assay buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate and 0.1% SDS) and protein content was measured using a standard BCA kit (Beyotime, P0009)

For electrophysiological studies, male pathogen-free mice at an age of 618 weeks were used for all experiments

Estimation of -cell mass by metabolic tests: necessary, but how sufficient

That makes sense since sleep is when your body does the most repair of tissue

[DOI] [PubMed] 422.Townsend PA, et al

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