Cell pellets were resuspended in 0.3 ml small-scale lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 1 mg ml 1 lysozyme, 0.75 mg ml 1 polymyxin B, 0.01 mg ml 1 DNase I) per well and incubated at 30 C, 300 rpm, 5 cm shaking diameter for 30 min
Ekstrapolacja tych dawek na ludzi, przy uyciu standardowych czynnikw konwersji allometrycznej (czynnik 6,2 dla szczurw), daje teoretyczne dawki rzdu 1,6 g/kg u ludzi
Statements Author contributions KW proposed the research
The femoral notch, or intercondylar notch, is the groove at the distal end of the femur through which the ACL passes
Yes, an unopened, factory-sealed vial is sterile and stable at room temperature during shipping
Most side effects from this type of glutathione tend to be mild, and often occur at the start of supplementation and disappear when the body gets used to it