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In a recent study, Zilocchi et al

The peptides anti-inflammatory effects offer pain relief and help promote tissue repair

Acute toxicity studies in animals, if extrapolated to humans, implicate a single dose of 10-15 grams necessary to produce significant liver toxicity, although several human case reports have shown toxicity at doses less than 4 grams per day (likely due to having one or more risk factors for sensitivity to toxicity) (Amar 2007)

If you are slightly over 12 hours, no biggie, take your patch off and be sure to give your body a 12 hour break before you apply the patch again the next day

Clinical human data is extremely limited

Timing considerations Most protocols recommend evening administration, 30-60 minutes before intended sleep