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Validation studies have been conducted in many countries, with proposed cutoff values ranging from 10 to 22 [104,105,106,107,108,109,110]

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A particularly aggressive form of lung cancer, ROS-positive NSCLC is difficult to treat and can often spread to the brain

Samples were then placed on a laser confocal microscope, excited with a 488 nm wavelength, and fluorescence signals were sequentially captured in optical slices to obtain spatial distribution within the cells and nuclei