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Targeting the APLN pathway with antagonists like ML221 has been shown to alleviate BTB damage and improve sperm quality in diabetic mice [54]

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At least five animals were then individually picked into a new Eppendorf tube with M9 buffer and homogenized using a motorized tissue grinder (Fisher Scientific) coupled with a 1.5 mL plastic pestle (Fisher Scientific)

doi: 10.3390/TOXICS13040322 158

Environmental pollution is a major contributor to oxidative stress in sperm