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Image acquiring was carried out using a Plan-APO 100/1.4 NA oil objective with the following filter set: BP 450488 nm, FT 495 nm, and BP 512542 nm

The cell lysate was clarified by centrifugation twice (10 minutes, 4C, 20,000 RPM) and 50 l was removed for derivatisation of thiols by addition of 1.5 l 1 M DTT, 1.5 l Mono-bromobimane and 45 l 1 M CHES pH 9

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