The necessity for endocytosis and recycling to dephosphorylate some GPCRs presumably depends on the affinity of arrestins for the agonist occupied receptor (Oakley et al., 1999, 2000)
Toxicology 149:4350 Thomas C, Mackey MM, Diaz AA, Cox DP (2009) Hydroxyl radical is produced via the Fenton reaction in submitochondrial particles under oxidative stress: implications for diseases associated with iron accumulation
This 30-second step prevents contamination that could make days of reconstituted peptide unusable
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Sensorgrams were analyzed using a global fit to the 1:1 Langmuir binding model
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