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Peptides were desalted using ZipTip -C18 Pipette Tips (Thermo Scientific) and were analyzed by an Orbitrap Fusion Tribrid coupled to a Nano-LC Proxeon 1000 equipped with an easy spray ion source (all from Thermo Scientific)

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This approach keeps training output high while protecting skeletal muscle mass under metabolic stress

Such findings suggest that MOTS-c might play a contributing role in healthy aging, especially in maintaining metabolic function and physical performance as organisms get older