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The final concentration of DMSO was 0.1% in all wells

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co-IP, inputs and SN samples were prepared with x1 Bolt LDS sample buffer (B0008, Thermo Scientific) containing x1 Bolt sample reducing agent (B0009, Thermo Scientific), heated for 5 min at 90C and loaded on a gradient electrophoresis gel (NW04120BOX, Invitrogen)

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In addition the risk of infertility and type 2 diabetes is reduced