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The eluted proteins were dialyzed against buffer A using an Amicon Ultra-15 device with a molecular cutoff of 10 kDa (Millipore, UFC901008) and concentrated to 0.5 ml

doi: 10.3389/fendo.2024.1528457 Received 14 November 2024 Accepted 30 December 2024 Published 21 January 2025 Volume 15 - 2024 Edited by Claire Joanne Stocker, Aston University, United Kingdom Reviewed by Wael Sidrak, Abou Seifein Diabetes & Endocrine Center, Egypt Frederic Illouz, CHU Angers, France Christine Trapp, Connecticut Childrens Medical Center, United States Updates Copyright 2025 Koceva, Mleku Kozamernik, Jane, Herman, Ferjan and Jensterle

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Combined with exercise therapy, both proved more effective than just exercise alone [53]