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It can also affect the gut microbiota, potentially jeopardizing the humanmicrobiota symbiotic relationship and making the microbiota more susceptible to the development of diseases such as cancer, thyroid dysfunction, and cardiovascular disorders [123]

This separation can be seen in Figure 4 along with the separation of C5-valeryl-L-carnitine, C5-isovaleryl-L-carnitine, and 2-methyl-butyryl-L-carnitine

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Stop solution (50 L) was added, and the OD value of each well was measured at 450 nm within 15 min

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